I’m having trouble analyzing these electrophoretic patterns?
I’m finishing a lab report for a biology class, and I’m not certain on what to do. I ran an SDS-PAGE on 5 seed extracts and got the Rm following results: corn- .963, navy pea- .457, black eyed pea- .438, kidney bean- .457, and lima bean- .609. Which seeds seem to be the most closely related to each other and why?
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One comment a "I’m having trouble analyzing these electrophoretic patterns?"
Strictly speaking, you can’t compare them that way. You have too many factors to consider. You can compare the structure of the seed, but you can’t compare the phylogeny of the plants you’re currently studying. If you wanted to do that, you’d be better off comparing DNA sequences of mitochondrial DNA isolated from these plants: a simple BLAST (local)comparison will do to get some idea of their relationship.
You do realize, however, that whoever made that excercise for you .was only going through the forms, and wasn’t really asking a question he/she didn’t already know the answer to
Off hand, you could probably conclude that similar band patterns among both the peas, as well as the kidney beans suggest a similar pattern of protein production, The band pattern itself wouldn’t be sufficient to conclusive prove this so.
This may or may not indicate that these seeds are making the same kind of protein. You’d need the amino acid sequence and an activity assay or two to make that conclusive. ..
So I’d say, declare the study as inconclusive pending further verification via sequencing and activity assays.
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